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cd4  (Bio-Rad)
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Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized <t>lymphocytes,</t> with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.
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Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized <t>lymphocytes,</t> with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.
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Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized <t>lymphocytes,</t> with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.
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Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized <t>lymphocytes,</t> with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.
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Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized <t>lymphocytes,</t> with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.
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Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized <t>lymphocytes,</t> with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.
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Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized <t>lymphocytes,</t> with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.
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Image Search Results


Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized lymphocytes, with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.

Journal: Veterinary Medicine and Science

Article Title: Rare Feline Gingival Extramedullary Plasmacytoma: Clinical Application of Electrochemotherapy for Local Control

doi: 10.1002/vms3.71294

Figure Lengend Snippet: Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized lymphocytes, with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.

Article Snippet: For flow cytometric analysis, antibodies against CD21 (B‐cell marker; clone CA2.1D6, RPE‐conjugated, Bio‐Rad Antibodies, MCA1781R), CD5 (pan–T‐cell marker; clone FE1.1B11, FITC‐conjugated, Bio‐Rad Antibodies, MCA2038F), CD4 (helper T‐cell marker; clone VPG34, FITC‐conjugated; Bio‐Rad Antibodies, MCA1346F) and CD8 (cytotoxic T‐cell marker; clone VPG9, RPE‐conjugated; Bio‐Rad Antibodies, MCA1347R) were used.

Techniques: Immunohistochemical staining, Staining, Expressing